Cytotoxicity, anti-adhesive and anti-angiogenic effects of Caspian Cobra snake (Naja oxiana) venom on human endothelial cells
نویسندگان
چکیده
Snake bite was known as one of the most important public health concerns world wild. Respiratory and cardiac problems and destruction of endothelium system occurs as a result of snake bite. Some proteins and peptides in snake venom have potential for drug discovery studies. According to the important role of cell adhesion and angiogenesis in cancer development, identification of new therapeutics that targets this process seems indispensable. Evaluation of cytotoxicity, anti-adhesive and anti-angiogenic effects of Caspian Cobra snake (Naja oxiana) venom on human endothelial cell was the main aim of current study. Methods: Crude Cobra snake venom was fractionated by fast protein liquid chromatography (FPLC) using S-200 column. Purity of crude venom and each fraction was monitored by SDS-PAGE. Cytotoxicity of Caspian Cobra snake (Naja oxiana) crude venom and fractions on human endothelial cells was evaluated by MTT assay Adhesion and tube formation
منابع مشابه
Evaluation of Anti-Cancer Effects of Caspian Cobra (Naja naja oxiana) Snake Venom in Comparison with Doxorubicin in HeLa Cancer Cell Line and Normal HFF Fibroblast
Introduction: Cancer is the leading cause of death in most countries. There are several methods used to treat cancer. Doxorubicin is one of the most important chemotherapy drugs that has several side effects, such as infertility, hyperuricemia, neuropathy, and cardiomyopathy. This study aimed to evaluate the anti-cancer effects of Naja naja oxiana in comparison with doxorubicin in Hela (Human c...
متن کاملAnticancer Activity a of Caspian Cobra (Naja naja oxiana) snake Venom in Human Cancer Cell Lines Via Induction of Apoptosis
Abstract Cancer is the leading cause of death worldwide. Current anticancer drugs involve various toxic side effects; efforts are ongoing to develop new anticancer agents especially from the screening of natural compounds. Present study investigated cytotoxic effects and mode of cell death induced by the Caspian cobra venom in some human cancer cell lines. Cytotoxic effects of snake venom toxin...
متن کاملAnticancer Activity a of Caspian Cobra (Naja naja oxiana) snake Venom in Human Cancer Cell Lines Via Induction of Apoptosis
Abstract Cancer is the leading cause of death worldwide. Current anticancer drugs involve various toxic side effects; efforts are ongoing to develop new anticancer agents especially from the screening of natural compounds. Present study investigated cytotoxic effects and mode of cell death induced by the Caspian cobra venom in some human cancer cell lines. Cytotoxic effects of snake venom toxin...
متن کاملAcute Effects of the Iranian Snake (Naja Naja Oxiana) Venom on Heart
The myocardial effect of snake venoms is considered as one of the most common pathogenesis in many cases of snake envenomation. This study was under taken to investigate the effects of snake (Naja naja oxiana) venom on cardiac function in experimental animals. The blood samples from all the rabbits were collected before venom injection and then 140 µg/kg venom of snake (Naja naja oxiana) was in...
متن کاملThe Effect of Snake Venom (Naja naja oxiana) on Proliferation Rate of cancer Cells
Aims Because cancer is the leading cause of death worldwide, finding a better way to treat it seems essential. Doxorubicin is one of the most common drugs in the treatment of cancer, which has many negative and toxic effects. Therefore, efforts to produce effective anticancer drugs through screening natural compounds, such as animal toxins continue. This study aimed to evaluate the effect of Na...
متن کامل